REGULARITIES OF LACTOSE HYDROLYSIS IN DAIRY RAW MATERIALS
DOI:
https://doi.org/10.31073/foodresources2020-14-17Keywords:
інтолерантність до лактози, кисломолочні низьколактозні продукти, ферментативний гідроліз, препарат β-галактозидази, глюкоза, галактозаAbstract
The problem of lactase deficiency is relevant for people of different ages and can reduce the consumption of dairy products. Reduction of lactose content is achieved in various ways, among which the most common is the use of enzyme preparations. The article presents the results of experimental studies on the patterns of enzymatic hydrolysis of lactose in different types of carbohydrate raw materials by enzyme preparations of fungal and yeast origin. The parameters of hydrolysis of lactose in milk and whey, which allow to choose the rational conditions for technological operations depending on the organization of technological processes in production. The development of technologies for low-lactose fermented milk products will expand the range of dairy products for people intolerant to lactose. Reducing the lactose content of demineralized whey will expand the possibility of its use in other foods. The need to control the conditions of technological operations to ensure effective hydrolysis of lactose and the activity of enzyme preparations is experimentally substantiated. Liquid chromatography was used to quantify the content of lactose, glucose and galactose in different types of raw materials - skim milk, sour and curd whey under different conditions of enzymatic hydrolysis of lactose by enzymatic preparations of fungal and yeast origin. The presence of nonspecific proteolytic activity was revealed, which leads to an increase in the content of non-protein nitrogenous substances and deterioration of organoleptic properties of serum hydrolyzed by the enzyme preparation of lactase of fungal origin. Reducing the lactose content and enriching the composition of fermented milk products with whey proteins increase their biological value.
Downloads
References
https://doi.org/10.31073/foodresources2020-14-17
Suchy, F. J. (2010). NIH consensus development conference statement: lactose intolerance and health. NIH consensus and state-of-the-science statements. 2, 1-27.
Corgneau, M. (2017). Recent advances on lactose intolerance: Tolerance thresholds and currently available answers. Critical reviews in food science and nutrition.15, P.3344-3356. doi:10.1080/10408398.2015.1123671
Khavkyn A., Zhyhareva N. (2009). Laktaznaia nedostatochnost. [Lactase deficiency]. Hastroеnteroloh. [Gastroenterologist].1, 78-82 [in Russian]
Ipatova M. (2013). Pervichnaya i vtorichnaya laktaznaya nedostatochnost. [Primary and secondary lactase deficiency]. Farmateka [ Farmateka]. 11, 41-44. [in Russian].
Nutrient-Rich Dairy Foodsand You. [Online]; IDFFastsheet, Desember, 2012. International Dairy Federation, Brussels. https://www.fil-idf.org/wp-content/uploads/2016/04/Nutrient-Rich-Dairy-Food-and-You-1.pdf (accessed Febr23, 2018).
Staudacher H., Lomer M., Anderson J., Barrett J., Muir J., Irving P., Whelan K. (2012). Fermentable carbohydrate restriction reduces luminal bifidobacteria and gastrointestinal symptoms in patients with irritable bowel syndrome. J. Nutr. 142, 1510–1518.
Novalin S, NeuhausW, KulbeKD (2005). A new in nova tive process to produce lactose-reduced ski milk. J Biotechnol 119, 212–218.
Harju M, Kallioinen H, Tossavainen O. (2012). Lactose hydrolysis and other conversion sin dairy products: technologi cala spects. Int Dairy J. 22, 104–109
Kyhel N. (2005). Zakvasochnyye kultury dlya fermentirovannykh molochnykh produktov: osnovnyye svoystva i vidy.[ Starter cultures for fermented dairy products: basic properties and types]. Molochna promyslovist [Dairy industry]. 1(16), 26-29. [in Russian].
Drewnowski, А. (2009). Nutrient density of dairy products. [Online]; IDF World Dairy Summit ‘United Dairy World 2009’ Berlin 20-24 September 2009. https://www.fil-idf.org/wp-content/uploads/2016/06/Drewnowski_Berlin-manuscript_100916.pdf (accessed Febr23, 2018).
Danylchuk T.N (2013) Nizkoloaktoznyye molochnyye produkty. Puti polucheniya [Lowactose dairy products. Ways to get]. Molochnaia promyshlennost [Dairy industry] 11, 41-42 [in Russian].
Hanyna V., Kalynyna L., Bolshakova E. (2002). β-galaktozidaznaya aktivnost molochnokislykh bakteriy i bifidobakteriy. [β-galactosidase activity of lactic acid bacteria and bifidobacteria]. Molochnaia promyshlennost [Dairy industry].8, 36-37. [in Russian].
Karasova, P., Spiwok, V., Mala, S., Kralova, B., Russell, N.J. (2002). Beta- galactosidase activity in psychrophic microorganisms and their potential use in food industry. Czech J Food Sci. 20, 43-47.
Saqib S., Akram, A., Halim, S., Tassaduq, R. (2017). Sources of β-galactosidase and its applications in food industry Biotech.7, 79.
Pivarnik L., Senecal A. (1995). Hydrolytic and transgalactosylic activities of commercial beta-galactosidase (lactase) in food processing. Adv Food Nutr Res. 38, 1–102.
Pohosian, A. (2006). Do pytannia fermentatyvnoho hidrolizu laktozy za dopomohoiu β-halaktozydazy [To the question of enzymatic hydrolysulactose by β-galactosidase ]. Molochna promyslovist [Dairy industry] 3(28), 44-45 [in Ukrainian].
Chagarovskaya, A. (2008) Molochnoye morozhenoye s gidrolizovannoy laktozoy [Hydrolyzed Lactose Milk Ice Cream]. Molochna promyslovist [Dairy industry] 5(48), 68-69 [in Russian].
Hnitsevych V., Yudina T., Honchar Yu. (2018). Tekhnolohiia napivfabrykativ na osnovi nyzkolaktoznoi molochnoi syrovatky ta miakoti harbuza [Semi-finished technology based on low-lactose whey and pumpkin pulp] Tovary i rynky [Goods and markets]. 4, 105-114 [in Ukrainian].
Kalinina E., Romanchuk I. (2005). Primeneniya fermenta laktazy dlya gidroliza molochnogo sakhara pri proizvodstve sgushchenogo obezzhirenogo moloka [The use of the lactase enzyme for hydrolysis of milk sugar in the production of condensed skim milk]. Zbirnyk naukovykh prats Luhan. nats. un-tu, seriia: tekhnichni nauky [Collection of scientific works of Lugan. nat. Universities Series: Technical Sciences]. 224-228. [in Russian].
Chagarovskiy, A., Pogosyan. A. (2006). Vliyaniye fermentativnogo gidroliza laktozy s pomoshchyu preparata β-galaktozidazy na organolepticheskiye i fiziko-khimicheskiye pokazateli moloka [The effect of enzymatic hydrolysis of lactose using the drug ß-galactosidase on the organoleptic and physico-chemical characteristics of milk]. Molochna promyslovist [Dairy industry]. 8(33), 32-35 [in Russian].
Sychevskyi, M., Romanchuk, I., & Minorova, A. (2019). Pererobka molochnoi syrovatky: perspektyvy v Ukraini [Whey processing: prospects in Ukraine]. Food Science and Technology. 13(4). https://doi.org/10.15673/fst.v13i4.1557.
Perevalova Yu. (2008). Kislomolochnyy nizkolaktoznyy product [Low-acid sour milk product]. Molochnaya promyshlennost [Dairy industry]. 7, 60-61. [in Russian].
Downloads
Published
How to Cite
Issue
Section
License
Licensing and Copyright
The collection provides immediate open access, ensuring that the content is freely available to the global community, facilitating a broader exchange of knowledge in accordance with the principles of open science.
By submitting an article to the editors of the collection, authors confirm that the manuscript has not been submitted to other publications and grant the journal the right of first publication in accordance with the terms of the Copyright Transfer Agreement.
Copyright. Authors retain full copyright to their articles without restriction and simultaneously grant the journal a non-exclusive license for publication, distribution, and indexing.
Licensing. The collection publishes articles under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) license.
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.uk
This license permits:
- copying and distributing the material in any medium or format;
- use of the material for educational and research purposes.
- Subject to the following conditions:
- attribution of the author, source, and license link;
- noncommercial use;
- no distribution of modified material (no derivatives).
First Publication Rights. Authors grant the collection “Food Resources” first publication rights, these allowing the collection to distribute the scientific material with attribution of authorship and the original publication in the collection.
Self-Archiving. Authors may upload any version of their articles (preprint, postprint, or published version) to institutional, disciplinary, or personal repositories without embargo, provided they indicate that the article was published in the collection "Food Resources" and include the CC BY-NC-ND license.
ISSN
ISSN 




