METHODOLOGICAL APPROACHES TO OPTIMIZING CULTIVATION CONDITIONS OF THE LIMOSILACTOBACILLUS FERMENTUM LF-02 STRAIN
DOI:
https://doi.org/10.31073/foodresources2025-25-07Keywords:
lactic acid bacteria, cultivation parameters, growth dynamics, Limosilactobacillus fermentum, biotechnologyAbstract
Subject. Lactic acid bacteria, particularly the Limosilactobacillus fermentum species, are important microorganisms used in the food industry. Studying and optimizing their cultivation conditions to achieve the maximum yield of viable biomass is a relevant task in biotechnological research. Purpose. To determine the optimal cultivation conditions for the L. fermentum LF-02 strain in order to obtain the highest amount of biomass. Methods. The study used the L. fermentum LF-02 strain, which was cultivated to select optimal parameters on standard MRS medium. The cultivation parameters included incubation time: 8, 10, and 12 hours; inoculum volume (% v/v): 1, 2.5, 5, 7.5, and 10; temperature,°C : 28, 30, 32, 34, 37; and initial pH of the medium ranging from 5.0 to 7.5 with an increment of 0.5. The growth of the strain was determined using the serial dilution plating method. To study the growth dynamics and main physiological-biochemical parameters under the selected optimal conditions, the strain growth was determined by serial dilution plating, pH was measured potentiometrically, the amount of reducing sugars was determined by the ferricyanide method, and the content of free amino nitrogen by the ninhydrin method. Results. The optimal cultivation conditions for L. fermentum LF-02 were determined, promoting the accumulation of the maximum biomass amount (9.20±0.03 CFU/cm3): incubation time – 10 hours, inoculum volume – 5%, temperature – 34°C, and medium pH – within the range of 6.0–7.0. Changes in cell count, pH, reducing sugars, and free amino nitrogen during cultivation under optimal conditions were analyzed. The specific growth rate of the strain was calculated as 0.410 ± 0.04 h⁻¹, the utilization rate of reducing sugars – 72.07±5.12%, and of free amino nitrogen – 26.71±3.56%. Scope of Results. The obtained results can be used for further optimization of nutrient media and the development of a biotechnology for biomass production of the L. fermentum LF-02 strain.
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