ANALYSIS OF FERMENTATION METHODS FOR BIOETHANOL PRODUCTION (REVIEW)
DOI:
https://doi.org/10.31073/foodresources2025-24-20Keywords:
bioethanol, corn grain, enzymatic hydrolysis, fermentation, wortAbstract
Subject. Corn grain. Research into methods of fermenting grain raw materials for bioethanol production. Purpose. Analysis of fermentation methods for bioethanol production Methods. Analytical. Results. The bioethanol production market is growing rapidly with the processing of sugar-, starch- and lignin-containing raw materials, as well as secondary products of food and beverage production. The use of new corn hybrids technologically affects the adjustment of the bioethanol production regime, reducing resource consumption and increasing the yield of bioethanol. This contributes to the development of cost-effective technologies for bioethanol production, which is a priority area for the development of the country's energy sector. Currently, the following methods used for the production of bioethanol by fermenting carbohydrates with free yeast cells: batch, continuous, batch with feeding. When using the batch fermentation method, a number of technological issues arise, such as the use of free cells for fermentation, inhibition of the substrate and product, imperfection of the inoculum preparation processes and cleaning of the reactor between batches, which leads to an increase in the duration of the process. Therefore, research efforts focused on improving the fermentation conditions to increase bioethanol production and reactor efficiency in the direction of using batch fermentation. In batch fermentation, the substrate added at a relatively constant rate or at frequent intervals, while equivalent amounts of enzyme removed, maintaining a constant volume. In this case, continuous fermenters can operate without interruption for weeks or months, which is acceptable for industrial production. The use of batch fermentation also contributes to an increased dry matter ontent of the wort. Scope of results. Alcoholic industry.
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References
World Bioenergy Association. WBA Global Bioenergy Statistics; 2017. Available from: www.worldbioenergy.org/global-bioenergy-statistics.
OECD/Food and Agriculture Organization of the United Nations. OECD-FAO Agricultural Outlook 2015. Paris, France: OECD Publishing; 2015. https://doi.org/1787/agr_outlook-2015-en
European Biofuels Technology Platform. Strategic research agenda 2010 update: Innovation driving sustainable biofuels. 2010. Available from: https://www.biofuelstp.eu/srasdd/SRA_2010_update_web.pdf.
van Thuijl E, Deurwaarder EP. European biofuels policies in retrospect. Petten, the Netherlands: Energy Research Centre of the Netherlands (ECN). 2006. Available from: https://www.ecn.nl/publications/PdfTetch.aspx?nr=ECN-C-06-016.
Prieur-Vernat A, His S. (2007). Biofuels worldwide. Panorama 2007. Rueil-Malmaison, France: IFP-Innovation Energy Einveroment. Available from: https://www.firp.ula.ve/archivos/material_web_4xx/07_IFP_Biofuels.pdf.
Li K, Liu S, Liu X. (2014). An overview of algae bioethanol production. Int. J. Energ Res.; Vol. 38(8):965–77. https://doi.org/10.1002/er.3164.
Festel G, Würmseher M, Rammer C, Boles E, Bellof M. (2013)/ Modelling production cost scenarios for biofuels and fossil fuels in Europe. Discussion paper №13–075. Mannheim, Germany: ZEW – Centre for European Economic Research. Available from: https://ftp.zew.de/pub/zew-docs/dp/dp13075.pdf.
Zhao L, Zhang X, Xu J, Ou X, Chang S, Wu M. (2015). Techno-economic analysis of bioethanol production from lignocellulosic biomass in China: Dilute-acid pretreatment and enzymatic hydrolysis of corn stover. Energies. Vol. 8(5):4096–117. https://doi.org/10.3390/en8054096.
Balat M, Balat H. (2009)/ Recent trends in global production and utilization of bio-ethanol fuel. Appl Energy.;Vol. 86(11):2273–82. https://doi.org/10.1016/j.apenergy.2009.03.015.
Mussatto SI, Dragone G, Guimarães PMR, Silva JPA, Carneiro LM, Roberto IC, et al. (2010)/ Technological trends, global market, and challenges of bio-ethanol production. Biotechnol Adv., Vol.28(6):817–30. https://doi.org/10.1016/j.biotechadv.2010.07.001.
Jobling S. (2004). Improving starch for food and industrial applications. Curr Opin Plant Biol. Vol.7(2):210–8. https://doi.org/10.1016/j.pbi.2003.12.001.
Linoj Kumar N.V., Dhavala P., Goswami A., Maithel S. (2006). Liquid biofuels in South Asia: resources and technologies. Asian Biotechnol Develop Rev. Vol. 8. P. 31–49.
Kumar P.N., Kansinee L., SaiM.V., Kumar K.P., Parichat N., Toan L.T., Natthiya B. (2025). Augmented ethanol production from alkali-assisted hydrothermal pretreated cassava peel waste. Energy Sources, Part A: Recovery, Utilization and Environmental Effects. Vol. 47 (1), P. 8243 – 8253. DOI 10.1080/15567036.2021.1928338.
Biorefineries Roadmap, German Federal Government action plans for the material and energetic utilisation of renewable raw materials. 2012. Available from: https://www.bmbf.de/pub/Roadmap_Biorefineries_eng.pdf.
Khanal S.K. (2008). Anaerobic biotechnology for bioenergy production: Principles and applications. Ames, IA, USA: Wiley-Blackwell; https://doi.org/10.1002/9780813804545.
Gunasekaran P. M., Banu. J, R. (2023). Indexing energy and cost of the pretreatment for economically efficient bioenergy generation. Frontiers in Energy. Vol. 10. DOI 10.3389/fenrg.2022.1060599.
Dutch Sustainable Development Group (DSD). Feasibility study on an effective and sustainable bio-ethanol production program by Least Developed Countries as alternative to cane sugar export, DSD group; study report on bio-ethanol production in LDC0s, the Netherlands, May 20, 2005. [cited; available from: /www. swilion.nl/documenten/DSD%20RapportS].
Solomon B.D., Barnes J.R., Halvorsen K.E. (2007)/ Grain and cellulosic ethanol: History, economics, and energy policy. Biomass Bi¬oenergy. Vol.31(6):416–25. https://doi.org/10.1016/j.biombioe.2007.01.023.
Mousdale D.M. (2008). Biofuels: Biotechnology, chemistry and sustainable development. Boca Raton, FL, USA: CRC Press.
Soccol C.R., Faraco V., Karp S., Vandenberghe L.P.S., Thomaz-Soccol V., Woiciechowski A., Pandey A. (2011). Lignocellulosic bioethanol: Current status and future perspectives. In: Pandey A. Larroche C., Ricke S.C., Dussap C.G., Gnansounou E., editors. Biofuels – Alternative feedstocks and conversion processes. Oxford, UK: Academic Press. P. 101–22. https://doi.org/10.1016/B978-0-12-385099-7.00005-X.
Lee S, Speight J.G., Loyalka S.K., editors. Handbook of alternative fuel technologies. Boca Raton, FL, USA: CRC Press; 2007. https://doi.org/10.1201/9781420014518.
Schubert C. (2006). Can biofuels finally take center stage? Nat Biotechnol. Vol.24:777–84. https://doi.org/10.1038/nbt0706-777.
Cardona C.A., Sanchez O.J. (2007). Fuel ethanol production: process design trends and integration opportunities. Bioresource Technol. Vol.98:2415–57.
Yue-Qin Tang, Ming-Zhe An, Ya-Ling Zhong, Morimura Shigeru, Xiao-Lei Wu, Kenji Kida (2010). Continuous ethanol fermentation from non-sulfuric acid-washed molasses using traditional stirred tank reactor and the flocculating yeast strain KF7. Journal of Bioscience and Bioengineering. Vol. 109, Issue 1, P. 41-46. https://doi.org/10.1016/j.jbiosc.2009.07.002
Jianliang Yu, Xu Zhang, Tianwei Tan (2007). An novel immobilization method of Saccharomyces cerevisiae to sorghum bagasse for ethanol productionJournal of Biotechnology. Vol. 129, Issue 3, P. 415-420. https://doi.org/10.1016/j.jbiotec.2007.01.039
Anuchit Rattanapan, Savitree Limtong, Muenduen Phisalaphong (2011). Ethanol production by repeated batch and continuous fermentations of blackstrap molasses using immobilized yeast cells on thin-shell silk cocoons. Applied Energy. Vol. 88, Issue 12, P. 4400-4404. https://doi.org/10.1016/j.apenergy.2011.05.020.
Muenduen Phisalaphong, Nuttapan Srirattana, Wiwut Tanthapanichakoon (2006). Mathematical modeling to investigate temperature effect on kinetic parameters of ethanol fermentation. Biochemical Engineering Journal. Vol. 28, Issue 1, P. 36-43. https://doi.org/10.1016/j.bej.2005.08.039.
Óscar J. Sánchez, Carlos A. Cardona (2008). Trends in biotechnological production of fuel ethanol from different feedstocks. J Bioresource Technology. Vol. 99, Issue 13, P. 5270-5295. https://doi.org/10.1016/j.biortech.2007.11.013.
García-Aparicio M.P., Oliva J.M., Manzanares P., Ballesteros M., Ballesteros I., González A., Negro M.J. (2011). Second-generation ethanol production from steam exploded barley straw by Kluyveromyces marxianus CECT 10875. Fuel. Vol. 90, Issue 4, P. 1624-1630. https://doi.org/10.1016/j.fuel.2010.10.052.
Gabriel S. Aruwajoye, Y. Sewsynker-Sukai, E.B. Gueguim Kana (2020). Valorisation of cassava peels through simultaneous saccharification and ethanol production: Effect of prehydrolysis time, kinetic assessment and preliminary scale up. Fuel. Vol. 278, р. 118351. https://doi.org/10.1016/j.fuel.2020.118351.
Cao, N.J., M.S. Krishnan, J.X. Du, C.S. Gong N.W.Y. Ho et al., (1996). Ethanol production from corn cob pretreated by the ammonia steeping process using genetically engineered yeast. Biotechnol. Lett., l18: 1013-1018. DOI:10.1007/BF00129723.
Shigechi H, Koh J, Fujita Y, Matsumoto T, Bito Y, Ueda M, et al. (2004). Direct production of ethanol from raw corn starch via fermentation by use of a novel surface-engineered yeast strain codisplaying glucoamylase and a-amylase. Appl Environ Microbiol. Vol.70: 5037–40.
Mojovic L, Nikolic S, Rakin M, Vukasinovic M. (2006). Production of bioethanol from corn meal hydrolyzates. Fuel. Vol.85:1750–5.
Singh V., Johnston D.B., Naidu K., Rausch K.D., Belyea R.L., Tumbleson M. (2005). Comparison of modified dry-grind corn processes for fermentation characteristics and DDGS composition. Cereal Chem. Vol.82(2):187–90.
Somavat P, Li Q, de Mejia EG, Liu W, Singh V. (2016). Coproduct yield comparisons of purple, blue and yellow dent corn for various milling processes. Ind Crops Prod. Vol.87:266–72.
Rausch K.D., Belyea R.L. (2006). The future of coproducts from corn processing. Appl Biochem Biotechnol. Vol.128(1):47–86.
Uthumporn U., Zaidul I.S., Karim A. (2010). Hydrolysis of granular starch at subgelatinization temperature using a mixture of amylolytic enzymes. Food Bioprod Process. Vol.88(1):47–54.
Wang P, Singh V, Xu L, Johnston DB, Rausch KD, Tumbleson M. (2005). Comparison of enzymatic (E-Mill) and conventional dry-grind corn processes using a granular starch hydrolyzing enzyme. Cereal Chem. Vol.82(6):734–8.
Singh V, Batie C.J., Aux G.W., Rausch K.D., Miller C. (2006). Dry-grind processing of corn with endogenous liquefaction enzymes. Cereal Chem. Vol.83(4):317–20.
Urbanchuk J.M., Kowalski D.J., Dale B., Kim S. (2009). Corn amylase: improving the efficiency and environmental footprint of corn to ethanol through plant biotechnology. AgBioForum. Vol.12:149–54.
Kumar D. Singh V. (2016). Dry-grind processing using amylase corn and superior yeast to reduce the exogenous enzyme requirements in bioethanol production. Biotechnol Biofuels Vol. 9:228. DOI 10.1186/s13068-016-0648-1.
Shihadeh J, Huang H, Rausch K, Tumbleson M, Singh V. (2013). Design of a vacuum flashing system for high-solids fermentation of corn. Trans ASABE. Vol.56(6):1441–7.
Shihadeh J.K., Huang H., Rausch K.D., Tumbleson M.E., Singh V. (2014). Vacuum stripping of ethanol during high solids fermentation of corn. Appl Biochem Biotechnol. Vol.173(2):486–500.
Taylor F, Kurantz M.J., Goldberg N., McAloon A.J., Craig J.C. (2010). Dry-grind process for fuel ethanol by continuous fermentation and stripping. Biotechnol Prog. Vol.16(4):541–7.
Wang S, Ingledew W, Thomas K, Sosulski K, Sosulski F. (1999). Optimization of fermentation temperature and mash specific gravity for fuel alcohol production. Cereal Chem. Vol.76(1):82–6.
Ramalingham A, Finn R. (1977). The vacuferm process: a new approach to fermentation alcohol. Biotechnol Bioeng. Vol.19(4):583–9.
Huang H, Qureshi N, Chen MH, Liu W, Singh V. (2015). Ethanol production from food waste at high solids content with vacuum recovery technology. J Agric Food Chem. Vol.63(10):2760–6.
Pandey A., Nigam P., Soccol C.R., Soccol V.T., Singh D., Mohan R. (2000). Advances in microbial amylases. Biotechnol Appl Biochem. Vol.31(Pt 2):135–52.
Shigechi H, Koh J, FujitaY, Matsumoto T, Bito Y, Ueda M, et al. (2004). Direct production of ethanol from raw corn starch via fermentation by use of a novel surface-engineered yeast strain codisplaying glucoamylase and α-amylase. Appl Environ Microbiol. Vol.70(8):5037–40. https://doi.org/10.1128/AEM.70.8.5037-5040.2004.
Huang H.J., Ramaswamy S., Tschirner U.W., Ramarao B.V.. A review of separation technologies in current and future biorefineries. Sep Purif Technol. Vol.62(1):1–21. https://doi.org/10.1016/j.seppur.2007.12.011.
Bothast R.J., Schlicher M.A. (2005). Biotechnological processes for conversion of corn into ethanol. Appl Microbiol Biotechnol. Vol.67(1):19–25. https://doi.org/10.1007/s00253-004-1819-8.
Cardona C.A., Sánchez Ó.J. (2007). Fuel ethanol production: Process design trends and integration opportunities. Bioresour Technol. Vol. 98(12):2415–57. https://doi.org/10.1016/j.biortech.2007.01.002.
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