SCHIZOPHYLLUM COMMUNE AS A PROMISING EXO-POLYGALACTURONASE PRODUCER

Authors

DOI:

https://doi.org/10.31073/foodresources2025-25-09

Keywords:

basidiomycetes, Schizophyllum, pectinolytic enzymes, enzymatic activity, polygalacturonase, surface cultivation, liquid nutrient media

Abstract

Subject. Juices are products with high nutritional and commercial value, but their production is complicated by the presence of pectic compounds. Exopolygalacturonases are often used to decompose such compounds. Among the potential producers of exopolygalacturonases, the basidiomycete Schizophyllum commune is promising, as it has a developed system of extracellular enzymes and is capable of degrading plant cell wall components. Purpose. The aim of the study was to investigate the synthesis of exopolygalacturonase by S. commune strains in submerged culture. Methods. The objects of research were three strains of S. commune(S. commune 335, S. commune 1759 and S. commune 1770), obtained from The Mushroom Culture Collection of the M.G. Kholodny Institute of Botany of the NAS of Ukraine. Cultivation was carried out in glucose-peptone-yeast, synthetic media and modified Norcrans medium in static mode at a temperature of 28±1°C for 14 days. At the end of cultivation, the biomass yield was determined gravimetrically; the amount of reducing substances and proteins was determined spectrophotometrically. Statistical data processing was performed using the Duncan test. Results. The highest activity was demonstrated by S. commune 1759 strain on Norcrans medium (494.4±72.9 units/dm3), while strains 335 and 1770 were characterized by lower activity. The maximum specific activity (6.48±0.26 units/gprotein) was also found in strain 1759, and the highest synthesis productivity was found in strain 335 on synthetic medium (1510.5±227.4 units/gBM). The lowest activity values were observed in glucose-peptone-yeast medium, while Norcrans medium caused the maximum values. Scope of the results. The results obtained indicate the need for further studies of strains S. commune 335 and S. commune 1759 to optimize the yield of exopolygalacturonase and its application in the food industry.

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Published

2025-12-22

How to Cite

Zubyk, P., Klechak, I., & Linovytska, V. (2025). SCHIZOPHYLLUM COMMUNE AS A PROMISING EXO-POLYGALACTURONASE PRODUCER. FOOD RESOURCES, 13(25), 83–90. https://doi.org/10.31073/foodresources2025-25-09

Issue

Vol. 13 No. 25 (2025): Food Resources

Section

Технічні науки

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