ANALYSIS OF THE GROWTH OF A LIMOSILACTOBACILLUS FERMENTUM STRAIN IN A GLUCOSE-MALTOSE NUTRIENT MEDIUM

Authors

DOI:

https://doi.org/10.31073/foodresources2025-24-08

Keywords:

lactic acid bacteria, nutrient medium, maltose, Limosilactobacillus fermentum, sugar metabolism

Abstract

Subject. Lactic acid bacteria (LAB) have a wide range of applications across various industrial sectors. Selecting an optimal nutrient medium is an important step in studying the growth and biomass production of LAB. One of the key carbohydrate components of nutrient media is maltose, which is used for cultivating many microorganisms. Therefore, research concerning the effectiveness of maltose as a carbohydrate source for LAB is relevant and may be applied to further medium optimization. Purpose. To investigate the growth characteristics of a L. fermentum strain on maltose-containing nutrient media and to evaluate the effect of maltose on the main kinetic parameters of the culture. Methods. A L. fermentum strain was used in this study and cultivated in maltose-containing nutrient media: M – with 2% (w/v) maltose, GM – with 1% (w/v) maltose and 1% (w/v) glucose, and the control medium G containing 2% (w/v) glucose. Biomass concentration was assessed by serial dilution plating on MRS agar; pH changes were measured potentiometrically; changes in reducing sugar content were determined using the ferricyanide method. Results. A positive effect of the GM and M media on strain growth was observed, with viable cell counts of 9.42 ± 0.09 log CFU/mL and 9.42 ± 0.01 log CFU/mL, respectively. Comparable pH values were observed across all experimenta consumption by the strain were recorded. The highest calculated specific growth rates were obtained in the M (0.65 ± 0.01 h-1) and GM (0.59 ± 0.06 h-1) media. Scope of Results. The results indicate the feasibility of using maltose as a primary or supplementary carbon source for cultivating L. fermentum and support the potential for incorporating maltose-containing components to optimize the nutrient medium.

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Published

2025-06-05

How to Cite

Khablenko, A., Danylenko, S., Dugan, O., & Bodnarchuk, O. (2025). ANALYSIS OF THE GROWTH OF A LIMOSILACTOBACILLUS FERMENTUM STRAIN IN A GLUCOSE-MALTOSE NUTRIENT MEDIUM. FOOD RESOURCES, 13(24), 74–82. https://doi.org/10.31073/foodresources2025-24-08

Issue

Vol. 13 No. 24 (2025): FOOD RESOURСES

Section

Технічні науки

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