SCREENING OF TRAMETES VERSICOLOR STRAINS AS POTENTIAL PRODUCERS OF PECTIN METHYL ESTERASE

Authors

DOI:

https://doi.org/10.31073/foodresources2024-23-07

Keywords:

basidiomycetes, Trametes, pectinolytic enzymes, enzymatic activity, pectin methyl esterase

Abstract

Objective. Juices are one of the most popular beverages in the world because they are not only tasty, but also contain many nutrients. Various enzymes are used for their production, primarily pectin methylesterases, which play an important role in the processing of raw materials, improving the quality and increasing the yield of the final product. Among the biotechnological objects that can synthesize these enzymes, a special place is occupied by basidiomycetes, such as Trametes versicolor. These fungi are capable of synthesizing pectin methyl esterase, which makes them promising for use in the food industry. The aim of the study was to investigate the potential of Trametes versicolor as a producer for the production of pectin methyl esterase. Methods. Three strains of T. versicolor (T . versicolor 353, T. versicolor 1689 and T. versicolor 5094) obtained from the Collection of Cap Fungi of the M.G. Kholodny Institute of Botany of the National Academy of Sciences of Ukraine were used as objects of study. Cultivation was carried out in glucose-peptone-yeast medium under static conditions at 28 ± 1 °C for 14 days. At the end of cultivation, the pH level was determined potentiometrically; biomass yield and amount of dry matter gravimetrically; content of reducing substances spectrophotometrically; pectin methylesterase activity titrimetrically. Statistical data processing was performed using Duncan's test. Results. The strain T. versicolor 353 was distinguished by the highest biomass yield (CBM = 5.96 ± 0.18 g/dm3) and the most effective consumption of reducing substances (ERS = 24.37 ± 0.82%). It was established that the level of consumption of dry matter of all strains was the same (EDS = 27.60–31.16%). T. versicolor 5094 strain showed the highest PME activity (251.11 ± 50.48 units/dm3) and synthesis productivity (υ = 35.17–55.44 units/gBM), which exceeds the indicators of T. versicolor 1689 strains (almost twice) and T. versicolor 353 (by 20 %).  Scope of the results. The results obtained indicate the need for further research aimed at optimizing the yield of pectin methyl esterase from T. versicolor 5094 strain and the use of this enzyme in industrial processes.

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Published

2024-12-25

How to Cite

Zubyk, P., & Klechak, I. (2024). SCREENING OF TRAMETES VERSICOLOR STRAINS AS POTENTIAL PRODUCERS OF PECTIN METHYL ESTERASE. FOOD RESOURCES, 12(23), 66–73. https://doi.org/10.31073/foodresources2024-23-07

Issue

Vol. 12 No. 23 (2024): FOOD RESOURСES

Section

Технічні науки

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