EFFECT OF MAGNETIC FIELD AND ENVIRONMENTAL PH ON THE ADSORPTION EFFICIENCY OF CELLS OF THE GENUS LACTOBACILLUS
DOI:
https://doi.org/10.31073/foodresources2023-20-02Keywords:
high-dispersed silica, lactobacillus, probiotics, adsorption, magnetic field, pH, viability, milk fermentation.Abstract
Subject. Adsorption of probiotic cultures Lactobacillus acidophilus, namely strains 27 and Narine, and Lactobacillus plantarum, on high-dispersed silica. Purpose. The purpose of this study was to investigate the influence of a magnetic field with intensities of 0.03 and 0.09 T and the pH of the medium on the efficiency of cell adsorption of Lactobacillus probiotic strains. Method. Microbiological and biotechnological methods were applied in this research. Hydrogels based on high-dispersed silica "Enterosgel" and "Toxin.NET" were used as adsorbents. Neodymium magnets with intensities of 0.03 T and 0.09 T were employed to create the magnetic field. The efficiency of adsorption was evaluated by the cell survival of probiotic cultures after adsorption on hydrogel preparations under the influence of the magnetic field and in the case of pH adjustment by adding acid or alkali. The functional properties of the obtained composite preparations were assessed by the time of clot formation after milk fermentation and its acidity. Results. The research results demonstrated that immobilized cells of Lactobacillus acidophilus on the hydrogel of methylsilicic acid had enhanced protection not only against harsh conditions of the gastrointestinal tract (low pH and bile influence) but also against magnetic fields with intensities of 0.03 T and 0.09 T, which had an overall negative impact on native (non-immobilized) cells. The functional activity of cells immobilized in this way on the hydrogel based on high-dispersed silica "Enterosgel," evaluated by acidity and clot formation time, was only slightly reduced: up to 4,3-11,3%. This confirms the assumption that the assessment of cell survival by the Koch's cup method does not fully correspond to the real state of the cells. It was also determined that conducting adsorption in the pH range below 4 is impractical, and the optimal conditions for L. plantarum adsorption were in the pH range of 6 to 7. Scope of results. The research findings can be utilized for the development of complex probiotic preparations based on high-dispersed silica, which may be appropriate for preserving the functionality of probiotic cells used for starter cultures or in the production of dietary supplements containing beneficial microorganisms.
Downloads
References
References
Dodoo, C. C., Wang, J., Basit, A. W., Stapleton, P., Gaisford, S. (2017). Targeted delivery of
probiotics to enhance gastrointestinal stability and intestinal colonisation. International Journal of
Pharmaceutics 530 (1 2), 224 229. https://doi.org/10.1016/j.ijpharm.2017.07.068.
Li, Z., Behrens, A. M., Ginat, N., Tzeng, S. Y., Lu, X., Sivan, S., Langer, R., Jaklenec, A. (2018).
Biofilm Inspired encapsulation of probiotics for the treatment of complex infections. Advanced
Materials 30 (51), e 1803925. https://doi.org/10.1002/adma.201 803925.
Geng, W., Jiang, N., Qing, G. Y., Liu, X., Wang, L., Busscher, H. J., Tian, G., Sun, T., Wang, L.
Y., Montelongo, Y., Janiak, C., Zhang, G., Yang, X. Y., & Su, B. L. (2019). Click reaction for reversible
encapsulation of single yeast cells. ACS Nano 13 (12), 14459 14467.
https://doi.org/10.1021/acsnano.9b08108
Danylenko S., Romanchuk I., Marynchenko L. (2021) Immobilization of probiotic cultures with
enterosorbents based on highly dispersed silica. Journal of Microbiology, Biotechnology and Food
Sciences, 11(2). https://doi.org/10.15414/jmbfs.3334.
Danylenko, S., Marynchenko, L., Bortnyk, V., Potemska, O., Nizhelska, O. (2022). Use of highly
dispersed silica in biotechnology of complex probiotic product based on bifidobacteria. Innovative
Biosystems and Bioengineering 6 (1), 16 24. https://doi.org/10.20535/ibb.2022.6.1.256179
Marynchenko L., Nizhelska O., Kurylyuk A., Naumenko S., Makara V. (2020) Observed effects
of electromagnetic fields action on yeast and bacteria cells attached to surfaces. 2020 IEEE 40th
International Conference on Electronics and Nanotechnolo gy (ELNANO) ELNANO), Kyiv, Ukraine, 22 24 April.
608. https://doi.org/ 10.1109/ELNANO50318.2020.9088883
Nizhelska, O., Marynchenko, L., Makara, V., Naumenko, S., & Kurylyuk, A. (2018). The
stabilizing effect of magnetic field for the shape of yeast cells saccharomyces cerevisiae on silicon
surface. Innovative Biosystems and Bioengineering 2 (4), 278 286.
ht tps://doi.org/ 10.20535/ibb.2018.2.4.151881
Wei, H., Geng, W., Yang, X. Y., Kuipers, J., van der Mei, H. C., Busscher, H. J. (2022).
Activation of a passive, mesoporous silica nanoparticle layer through attachment of bacterially derived
carbon quantum dots for protection and functional enhancement of probiotics. Materials Today Bio
http s://doi.org/10.1016/j.mtbio.2022.100293
Jesionowski, T., Krysztafkiewicz, A. (1999). Properties of highly dispersed silicas precipitated in
an organic medium. Journal of Dispersion Science and Technology 20 (6), 1609 1623.
https://doi.org/10.1016/j.mtbio.2022.10029
Pope, E. J. (1995). Gel encapsulated microorganisms: Saccharomyces cerevisiae Silica gel
biocomposites. Journal of Sol Gel Science and Technology, 4, 225 229.
https://doi.org/10.1007/BF00488377.
Campostrini, R., Carturan, G., Caniato, R., Piovan, A., Filippini, R., Innocenti, G.,
Cappelletti, E. M. (1996). Immobilization of plant cells in hybrid sol gel materials. Journal of Sol Gel
Science and Technology 7 (1 2), 87 97. https://doi.org/10.1007/BF00401888
Irkitova, A. N., Matsyura, A. V. (2017). Ecological and biological characteristics of
Lactobacillus acidophilus . Ukrainian Journal of Ecology, 7(4), 214 230.
https://doi.org/10.15421/2017_109.
Brányik, T., Kuncová, G., Páca, J., Demnerová, K. (1998). Encapsulation of microbial cells into
silica gel. Journal of Sol Gel Science and Technology, 13, 283 287.
https://doi.org/10.1023/A:10 08655623452
Downloads
Published
How to Cite
Issue
Section
License
Licensing and Copyright
The collection provides immediate open access, ensuring that the content is freely available to the global community, facilitating a broader exchange of knowledge in accordance with the principles of open science.
By submitting an article to the editors of the collection, authors confirm that the manuscript has not been submitted to other publications and grant the journal the right of first publication in accordance with the terms of the Copyright Transfer Agreement.
Copyright. Authors retain full copyright to their articles without restriction and simultaneously grant the journal a non-exclusive license for publication, distribution, and indexing.
Licensing. The collection publishes articles under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) license.
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.uk
This license permits:
- copying and distributing the material in any medium or format;
- use of the material for educational and research purposes.
- Subject to the following conditions:
- attribution of the author, source, and license link;
- noncommercial use;
- no distribution of modified material (no derivatives).
First Publication Rights. Authors grant the collection “Food Resources” first publication rights, these allowing the collection to distribute the scientific material with attribution of authorship and the original publication in the collection.
Self-Archiving. Authors may upload any version of their articles (preprint, postprint, or published version) to institutional, disciplinary, or personal repositories without embargo, provided they indicate that the article was published in the collection "Food Resources" and include the CC BY-NC-ND license.
ISSN 
ISSN 
