INTENSIFICATION OF THE YEAST GROWING PROCESS BY DISCRETE-IMPULSE PROCESSING OF CULTURAL MEDIA

Authors

DOI:

https://doi.org/10.31073/foodresources2022-19-13

Keywords:

discrete-pulse input of energy, mass transfer, absorption, microorganisms, cultivation, intensification, cultural media

Abstract

Subject of research. Solving the problem of providing yeast cells with a sufficient amount of dissolved oxygen to activate growth and reproduction throughout the entire cultivation period. Purpose. The intensification of the process of aeration of cultural environments by the method of discrete-pulse energy input, implemented in a rotor-pulsation apparatus. Methods. The process of aeration of culture media in the technology of growing Saccharomyces cerevisiae yeast by the method of discrete-pulse energy input was studied. The rate of oxygen absorption in the culture liquid was determined by the amount of yeast biomass grown during the cultivation period. Results. In the course of experiments on the cultivation of yeast on molasses solutions, the dependence of the oxygen mass transfer rate on the angular speed of rotation of the rotor unit in culture media with a dry solid content of 3-10% was determined. With a decrease in the content of solid content from 10 to 5% when processing with an angular speed of 48 rpm, the oxygen absorption rate of the culture liquid increases by 1.9 times. With an increase in the frequency of pulsations from 2 to 3.85 kHz, mass transfer increases from 5 to 7.3 g/l∙h at solid content of 3% and from 2 to 4 g/∙h at solid content of 10%. A further increase in the frequency of pulsations leads to the inactivation of part of the yeast cells. It was also found that the optimal values of the shear rate of the flow is 90-100∙103s-1. Application of the method of discrete-pulse energy input in absorption technologies makes it possible to significantly intensify mass exchange processes. Scope of research results. Oxygen saturation of microorganisms can be used to grow yeast biomass for the bakery and alcohol industries.

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References

Pepper, Ian. L., Gerba, C. P. (2015). Environmental Microbiology (Third edition). Elsevier,

Amsterdam, 705.

Mounsef, J., Salameh, D., Louka, N., Brandam, C, Lteif, R. (2015). The effect of aeration conditions,

characterized by the volumetric mass transfer coefficient KLa, on the fermentation kinetics of Bacillus

thuringiensis kurstaki, Chemical Engineering Science, V. 210. 100-106.

Xu, C., Won, Yun J. (2004). Influence of aeration on the production and the quality of the

exopolysaccharides from Paecilomyces tenuipes C240 in a stirred-tank fermenter. Enzyme and Microbial

Technology. V. 210, 1. 33-39.

Druzinec, D., Salzig, D., Kraume, M., Czermak, P. (2015). Micro-bubble aeration in turbulent stirred

bioreactors: Coalescence behavior in Pluronic F68 containing cell culture media. Chemical Engineering

Science. V. 126. 160-168.

Ghasem, D. Najafpour. (2007). Biochemical engineering and biotechnology. Elsevier, Amsterdam.

p. 650.

Dolinskiy, А., Ivanitskiy, G. (2008). Teplomassoperenos i gidrodinamika v parozhidkostnikh

sredakh. Teplovie osnovi diskretnoi vkhodnoi impulsnoi energii. [Heat and mass transfer and hydrodynamics

in the vapor-liquid media. Thermal basics of discrete input pulse energy]. Naukova dumka [Scientific thought].

Kyiv. 234 [in russian].

Grabova, T. (2007). Dispergirovanie geterogennikh sistem v rotorno-pulsatsionnikh apparatakh

diskovo-tsilindricheskogo tipa: dis. kand. tekhn. nauk [Dispersion of heterogeneous systems in a rotarypulsation apparatus of disc-cylindrical type], PhD dissertation (Engineering). Іnstytut tekhnycheskoi

teplofyzyky [Institute of engineering thermophysics]. 221. [in russian].

Dolinski, A. (2015). Mikro- i nano- urovnevie protsesi v tekhnologiyakh DIVE [Micro and nano

leveled processes DPIE technologies]. Akademperiodika [Academic periodical]. Кyiv. 422-437 [in russian].

Dolinski, A. Obodovich, A., Borhalenko, Yu. (2012). Metod vvoda diskretnoi impulsnoi energii i

yego realizatsiya. [The method of discrete pulse energy input and its implementation]. Apostrof [Apostrophe].

Kharkiv. 156 [in russian].

Pauline, M. Doran. (2013). Bioprocess Engineering Principles (Second Edition). Elsevier. 761-852.

Bessarab, О., Obodovich, О., Sydorenko, V. (2016). Intensification of mass transfer processes in

gas-liquid media by discrete-pulse energy input method. Ukrainian Food Journal. Volume 5. Issue 2. 368-375.

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Published

2022-12-25

How to Cite

Obodovych, O., Sydorenko, V., & Sheiko, T. (2022). INTENSIFICATION OF THE YEAST GROWING PROCESS BY DISCRETE-IMPULSE PROCESSING OF CULTURAL MEDIA. FOOD RESOURCES, 10(19), 116–122. https://doi.org/10.31073/foodresources2022-19-13

Issue

Vol. 10 No. 19 (2022): FOOD RESOURСES

Section

Технічні науки

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