FERMENTATION OF VICIA FABA BEAN PROTEIN CONCENTRATE: PERSPECTIVES OF USE IN PLANT-BASED BEVERAGE TECHNOLOGY

Authors

DOI:

https://doi.org/10.31073/foodresources2026-26-23

Keywords:

functional product, starter culture, fermentation, fermented product, plant-based beverages, protein concentrate, legume

Abstract

Subject. The problem of global food systems transformation and the search for new complete sources of plant protein in functional product technologies is considered. The advantages of using domestic legume raw materials Vicia faba L. and its processing products are analyzed, alongside the methods of overcoming biotechnological barriers associated with the presence of antinutrients and a specific legume off-flavor through targeted lactic acid fermentation. Purpose. To substantiate and investigate the technological conditions for fermentation of protein concentrate from Vicia faba L. beans bean protein concentrate to develop new fermented plant-based dairy alternatives with improved physicochemical, nutritional, and functional properties. Methods. Methods of enzymatic hydrolysis of starch impurities in the suspension using thermostable α-amylase with a comparative analysis of conventional thermal and microwave heating were applied and fermentation method using lactic acid starter cultures. Physicochemical transformations were evaluated by the kinetics of reducing sugars accumulation, changes in titratable acidity, and the accumulation of free amino nitrogen. Organoleptic characteristics were studied by the expert evaluation method using a developed 20-point scale. Results. It is proved that preliminary enzymatic treatment of the suspension prevents starch gelatinization and reduces the viscosity of the system. A high adaptability of lactic acid bacteria to the plant substrate is established, which is manifested in lowering the pH to 4.7, active sugar consumption by microorganisms, and intensification of proteolysis. Rational multicomponent starter cultures (Iprovit-BTA, Iprovit-Symbilact, Iprovit-Yogurt) are determined, ensuring the formation of a homogeneous yogurt-like structure and complete neutralization of the undesirable legume flavor and aroma. Scope of results. The research results are intended for use in the food industry for developing innovative functional products, diversifying the range of plant-based milk alternatives, and implementing technologies for deep processing of local legume crops.

Downloads

Download data is not yet available.

References

1. Sharma, N., Yeasmen, N., Dube, L., & Orsat, V. (2024). A review on current scenario and key challenges of plant-based functional beverages. Food Bioscience, 60, Article 104320. https://doi.org/10.1016/j.fbio.2024.104320.

2. Jiang, L., Lu, Y., & He, Q. (2025). Unlocking the microbial mechanisms in fermented bean products: advances and future directions in multi-omics and machine learning approaches. Critical Reviews in Food Science and Nutrition, 1–23. https://doi.org/10.1080/10408398.2025.2544764.

3. Ayala-Rodríguez, V. A., López-Hernández, A. A., López-Cabanillas Lomelí, M., González-Martínez, B. E., & Vázquez-Rodríguez, J. A. (2022). Nutritional quality of protein flours of fava bean (Vicia faba L.) and in vitro digestibility and bioaccesibility. Food Chemistry: X, 14, Article 100303. https://doi.org/10.1016/j.fochx.2022.100303.

4. Ahmad, I., Hao, M., Li, Y., Jianyou, Z., Yuting, D., & Lyu, F. (2022). Fortification of yogurt with bioactive functional foods and ingredients and associated challenges – A review. Trends in Food Science & Technology, 129, 558–580. https://doi.org/10.1016/j.tifs.2022.11.003.

5. Mayer Labba, I.-C., Frøkiær, H., & Sandberg, A.-S. (2021). Nutritional and antinutritional composition of fava bean (Vicia faba L., var. minor) cultivars. Food Research International, 140, Article 110038. https://doi.org/10.1016/j.foodres.2020.110038.

6. Paul, A. A., Kumar, S., Kumar, V., & Sharma, R. (2019). Milk Analog: Plant based alternatives to conventional milk, production, potential and health concerns. Critical Reviews in Food Science and Nutrition, 60(18), 3005–3023. https://doi.org/10.1080/10408398.2019.1674243

7. Tangyu, M., Muller, J., Bolten, C. J., & Wittmann, C. (2019). Fermentation of plant-based milk alternatives for improved flavour and nutritional value. Applied Microbiology and Biotechnology, 103(23-24), 9263–9275. https://doi.org/10.1007/s00253-019-10175-9.

8. Institute of Food Resources of NAAS of Ukraine. (2010). Kultury zakvashuvalni sukhi ta ridki [Dry and liquid starter cultures] (TU U 15.5-00419880-10:2010). Kyiv.

9. Dhull, S. B., Kidwai, M. K., Noor, R., Chawla, P., & Rose, P. K. (2021). A review of nutritional profile and processing of faba bean (Vicia faba L.). Legume Science, 4(3), Article e129. https://doi.org/10.1002/leg3.129.

10. Technical Committee, A. (2011). Free Amino Nitrogen (International Method). ASBC Methods of Analysis. American Society of Brewing Chemists. https://doi.org/10.1094/asbcmoa-wort-12.

11. Millar, K. A., Gallagher, E., Burke, R., McCarthy, S., & Barry-Ryan, C. (2019). Proximate composition and anti-nutritional factors of fava-bean (Vicia faba), green-pea and yellow-pea (Pisum sativum) flour. Journal of Food Composition and Analysis, 82, Article 103233. https://doi.org/10.1016/j.jfca.2019.103233.

12. Dehtiar, V. V., Radchenko, A. E., Grynchenko, N. H., & Grynchenko, O. O. (2024). Tekhnolohichni ta ekonomichni aspekty zastosuvannia bobovykh v tekhnolohiiakh kharchovoi produktsii: mini-ohliad [Technological and economic aspects of using legumes in food technology: a mini-review]. Journal of Chemistry and Technologies, 31(4), 886–896. https://doi.org/10.15421/jchemtech.v31i4.287753 [In Ukrainian].

Published

2026-06-30

How to Cite

Hrabovska, O. (2026). FERMENTATION OF VICIA FABA BEAN PROTEIN CONCENTRATE: PERSPECTIVES OF USE IN PLANT-BASED BEVERAGE TECHNOLOGY. FOOD RESOURCES, 14(26), 235–245. https://doi.org/10.31073/foodresources2026-26-23

Issue

Section

Технічні науки