THE USE OF LACTIPLANTIBACILLUS PLANTARUM AND LENTILACTOBACILLUS BUCHNERI LACTIC ACID BACTERIA TO PRODUCE CHICKPEA STARTER CULTURE IN BAKERY PRODUCT MANUFACTURING

Authors

DOI:

https://doi.org/10.31073/foodresources2026-26-12

Keywords:

нутова закваска, молочнокислі бактерії, Lactiplantibacillus plantarum, Lentilactobacillus buchneri, бродіння, хлібобулочні вироби

Abstract

Subject. Due to its high content of protein, dietary fiber, and biologically valuable compounds, chickpea flour is a promising raw material for improving the nutritional and biological value of bakery products. Lactic acid fermentation using selected strains of lactic acid bacteria is an effective approach to enhancing its technological properties. Purpose. To investigate the influence of L. plantarum and L.buchneri on the quality characteristics of chickpea sourdough and bakery products. Methods. Pure cultures of L. plantarum and L. buchneri obtained from the Ukrainian Collection of Microorganisms of the D.K. Zabolotny Institute of Microbiology and Virology of the National Academy of Sciences of Ukraine were used for the preparation of chickpea sourdoughs. The sourdoughs and bakery products were evaluated using physicochemical and sensory analyses. Volatile aromatic compounds were determined by gas chromatography–mass spectrometry. Results. The studied lactic acid bacteria provided active fermentation of chickpea flour within 14–16 h, resulting in sourdoughs with high lactic acid bacterial activity (23–44 min). The sourdough fermented with L. buchneri was characterized by an alcohol–ester profile, with ethanol (≈38.7%) and ethyl acetate (≈18.1%) as the predominant compounds. In contrast, L. plantarum promoted the formation of lactic and legume-like notes, with acetoin accounting for approximately 11.3% of the volatile compounds. The mixed culture produced the highest content of acetic acid (≈31.5%) and the most complex aromatic profile. The use of chickpea flour in sourdough reduced the beany flavor of bread. The combination of homofermentative and heterofermentative strains (L. plantarum and L. buchneri) proved more effective than monocultures. Owing to the optimal balance between gas-producing and gas-retaining capacities of the dough, bread produced with the mixed-culture sourdough exhibited the highest specific volume among all tested samples, exceeding the control by 12%. Practical implications. The obtained results confirm the potential of chickpea sourdoughs for application in breadmaking.

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References

1. Buialska, N. P., Humeniuk, O. L., Denysova, N. M., & Cheliabiieva, V. M. (2020). Pidvyshchennia kharchovoi tsinnosti khlibobulochnykh i boroshnianykh kondyterskykh vyrobiv: Monohrafiia [Increasing the nutritional value of bakery and flour confectionery products: Monograph]. Chernihiv, Ukraine: ChNTU. [in Ukrainian].

2. Guiné, R. P. F., & Florença, S. G. (2024). Development and Characterisation of Functional Bakery Products. Physchem, 4 (3), 234–257. https://doi.org/10.3390/physchem4030017.

3. Sadohara, R., Winham, D. M., & Cichy, K. A. (2022). Food Industry Views on Pulse Flour–Perceived Intrinsic and Extrinsic Challenges for Product Utilization. Foods, 11(14), 2146. https://doi.org/10.3390/foods11142146.

4. Wang, J., Li, Y., Li, A., Liu, R. H., Gao, X., Li, D., Kou, X., & Xue, Z. (2021). Nutritional constituent and health benefits of chickpea (Cicer arietinum L.): A review. Food Research International, 150, 110790. https://doi.org/10.1016/j.foodres.2021.110790.

5. Santos, F. G., Fratelli, C., Muniz, D. G., & Capriles, V. D. (2018). Mixture Design Applied to the Development of Chickpea-Based Gluten-Free Bread with Attractive Technological, Sensory, and Nutritional Quality. Journal of Food Science, 83(1), 188–197. https://doi.org/10.1111/1750-3841.14009.

6. Boukid, F., Zannini, E., Carini, E., & Vittadini, E. (2019). Pulses for bread fortification: A necessity or a choice? Trends in Food Science & Technology, 88, 416–428. https://doi.org/10.1016/j.tifs.2019.04.007.

7. Ouazib, M., Dura, A., Zaidi, F., & Rosell, C. (2016). Effect of Partial Substitution of Wheat Flour by Processed (Germinated, Toasted, Cooked) Chickpea on Bread Quality. International Journal of Agricultural Science and Technology, 4 (1), 8–18. https://doi.org/10.12783/ijast.2016.0401.02.

8. Mohammed, I., Ahmed, A. R., & Senge, B. (2012). Dough rheology and bread quality of wheat–chickpea flour blends. Industrial Crops and Products, 36 (1), 196–202. https://doi.org/10.1016/j.indcrop.2011.09.006.

9. Kotsiou, K., Sacharidis, D.-D., Matsakidou, A., Biliaderis, C. G., & Lazaridou, A. (2022). Physicochemical and functional aspects of composite wheat-roasted chickpea flours in relation to dough rheology, bread quality and staling phenomena. Food Hydrocolloids, 124, 107322. https://doi.org/10.1016/j.foodhyd.2021.107322.

10. Kaur, R., & Prasad, K. (2021). Technological, processing and nutritional aspects of chickpea (Cicer arietinum) – A review. Trends in Food Science & Technology, 109, 448–463. https://doi.org/10.1016/j.tifs.2021.01.044.

11. Kahala, M., Mäkinen, S., & Pihlanto, A. (2021). Impact of Fermentation on Antinutritional Factors. У Bioactive Compounds in Fermented Foods (с. 185–206). CRC Press. https://doi.org/10.1201/9780429027413-10.

12. Arora, K., Ameur, H., Polo, A., Di Cagno, R., Rizzello, C. G., & Gobbetti, M. (2021). Thirty years of knowledge on sourdough fermentation: A systematic review. Trends in Food Science & Technology, 108, 71–83. https://doi.org/10.1016/j.tifs.2020.12.008.

13. Gobbetti, M., Minervini, F., Pontonio, E., Di Cagno, R., & De Angelis, M. (2016). Drivers for the establishment and composition of the sourdough lactic acid bacteria biota. International Journal of Food Microbiology, 239, 3–18. https://doi.org/10.1016/j.ijfoodmicro.2016.05.022.

14. De Vuyst, L., Van Kerrebroeck, S., Harth, H., Huys, G., Daniel, H. M., & Weckx, S. (2014). Microbial ecology of sourdough fermentations: Diverse or uniform? Food Microbiology, 37, 11–29. https://doi.org/10.1016/j.fm.2013.06.002.

15. Lima, T. T. M., Hosken, B. d. O., Lindner, J. D. D., Menezes, L. A. A., Pirozzi, M. R., & Martin, J. G. P. (2023). How to deliver sourdough with appropriate characteristics for the bakery industry? The answer may be provided by microbiota. Food Bioscience, 103072. https://doi.org/10.1016/j.fbio.2023.103072.

16. Curiel, J. A., Coda, R., Centomani, I., Summo, C., Gobbetti, M., & Rizzello, C. G. (2015). Exploitation of the nutritional and functional characteristics of traditional Italian legumes: The potential of sourdough fermentation. International Journal of Food Microbiology, 196, 51–61. https://doi.org/10.1016/j.ijfoodmicro.2014.11.032.

17. Gobbetti, M., De Angelis, M., Di Cagno, R., Calasso, M., Archetti, G., & Rizzello, C. G. (2019). Novel insights on the functional/nutritional features of the sourdough fermentation. International Journal of Food Microbiology, 302, 103–113. https://doi.org/10.1016/j.ijfoodmicro.2018.05.018.

18. Rizzello, C. G., Coda, R., & Gobbetti, M. (2017). Use of Sourdough Fermentation and Nonwheat Flours for Enhancing Nutritional and Healthy Properties of Wheat-Based Foods. Fermented Foods in Health and Disease Prevention (с. 433–452). Elsevier. https://doi.org/10.1016/b978-0-12-802309-9.00018-2.

19. Chiacchio, M. F., Tagliamonte, S., Pazzanese, A., Vitaglione, P., & Blaiotta, G. (2025). Lactic acid fermentation improves nutritional and functional properties of chickpea flours. Food Research International, 115899. https://doi.org/10.1016/j.foodres.2025.115899.

20. Sáez, G. D., Sabater, C., Fara, A., & Zárate, G. (2021). Fermentation of chickpea flour with selected lactic acid bacteria for improving its nutritional and functional properties. Journal of Applied Microbiology. https://doi.org/10.1111/jam.15401.

21. Di Biase, M., Bavaro, A. R., Lonigro, S. L., Pontonio, E., Conte, A., Padalino, L., Minisci, A., Lavermicocca, P., & Valerio, F. (2019). Lactobacillus plantarum ITM21B fermentation product and chickpea flour enhance the nutritional profile of salt reduced bakery products. International Journal of Food Sciences and Nutrition, 70 (6), 701–713. https://doi.org/10.1080/09637486.2019.1567699.

22. Drobot, V. I. (2019). Dovidnyk z tekhnolohii khlibopekarskoho vyrobnytstva [Handbook of Bakery Production Technology]. Kyiv : ProfKnyha [in Ukrainian].

23. Drobot, V. I. (Ed.). (2015). Tekhnokhimichnyi kontrol syrovyny ta khlibobulochnykh i makaronnykh vyrobiv : navchalnyi posibnyk [Technochemical Control of Raw Materials and Bakery and Pasta Products: A Textbook ]. Kyiv : Kondor-Vydavnytstvo. [in Ukrainian].

24. Nouska, C., Hatzikamari, M., Matsakidou, A., Biliaderis, C. G., & Lazaridou, A. (2023). Enhancement of Textural and Sensory Characteristics of Wheat Bread Using a Chickpea Sourdough Fermented with a Selected Autochthonous Microorganism. Foods, 12 (16), 3112. https://doi.org/10.3390/foods12163112.

25. Seis Subaşı, A., & Ercan, R. (2024). Technological characteristics of whole wheat bread: effects of wheat varieties, sourdough treatments and sourdough levels. European Food Research and Technology, 250, 2593–2608. https://doi.org/10.1007/s00217-024-04560-6.

26. Huang, C., Zhang, B., Huang, J., Liu, Y., Chen, C., Omedi, J. O., Liang, L., Zhou, Z., Huang, W., & Li, N. (2024). The Effects of Single- or Mixed-Strain Fermentation of Red Bean Sourdough, with or without Wheat Bran, on Bread Making Performance and Its Potential Health Benefits in Mice Model. Foods, 13(17), 2856. https://doi.org/10.3390/foods13172856.

Published

2026-06-30

How to Cite

Bondarenko, Y., & Godunko, I. (2026). THE USE OF LACTIPLANTIBACILLUS PLANTARUM AND LENTILACTOBACILLUS BUCHNERI LACTIC ACID BACTERIA TO PRODUCE CHICKPEA STARTER CULTURE IN BAKERY PRODUCT MANUFACTURING. FOOD RESOURCES, 14(26), 134–146. https://doi.org/10.31073/foodresources2026-26-12

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Технічні науки